Stimulation of Sterol Synthesis in Peripheral Leukocytes of Leukemic Mice1
نویسندگان
چکیده
Bone marrow cells and peripheral blood leukocytes from leukemic AKR/J mice synthesize sterols from acetate at a vastly greater rate than the respective cells from normal AKR/J mice, i.e., approximately 20and 100-fold. The controlling enzyme of sterol synthesis, 3-hydroxy-3-melhylglutaryl coenzyme A reducÃ-ase, is readily detectable in gradient-purified leukocytes from leukemic mice, whereas normal leukocytes, fractions of red blood cells, and platelets express very low activity. The increased rate of sterol synthesis in leukocytes and bone marrow cells of leukemic animals does not result in an elevated plasma cholesterol level.
منابع مشابه
Stimulation of sterol synthesis in peripheral leukocytes of leukemic mice.
Bone marrow cells and peripheral blood leukocytes from leukemic AKR/J mice synthesize sterols from acetate at a vastly greater rate than the respective cells from normal AKR/J mice, i.e., approximately 20and 100-fold. The controlling enzyme of sterol synthesis, 3-hydroxy-3-melhylglutaryl coenzyme A reducÃ-ase, is readily detectable in gradient-purified leukocytes from leukemic mice, whereas nor...
متن کاملA comparison of delipidated sera used in studies of sterol synthesis by human mononuclear leukocytes.
Sterol synthesis in human mononuclear leukocytes is stimulated by delipidated serum. Synthesis in media containing serum delipidated by three different methods is compared. Significant differences between subjects are shown and the differences are maximized by measuring synthesis in serum delipidated by extraction with butanol-diisopropyl ether 40:60 and diethyl ether.
متن کاملInteracting cell populations in cultures of leukocytes from normal or leukemic peripheral blood.
Kinetic studies in cultures containing 2 X 10(5) peripheral leukocytes from patients with acute myelobastic leukemia revealed extensive, radiation-sensitive increases in thymidine incorporation without parallel increases in cell number. Modest and variable stimulation of 3HTdR incorporation was seen with the addition of either leukocyte-conditioned medium prepared with phytohemagglutin (PHA) or...
متن کاملRelationship between sterol synthesis and DNA synthesis in phytohemagglutinin-stimulated mouse lymphocytes.
Incubation of peripheral blood or isolated lymphocytes of C57L/J mice with phytohemagglutinin stimulated the incorporation of thymidine into DNA of lymphocytes as they transformed into large lymphoblasts. DNA synthesis began after about 24 hr of incubation and reached a peak at 48 hours. The de-novo synthesis of sterols from acetate was stimulated much earlier, at 4 hr of incubation, and the ra...
متن کاملNon-sterol regulation of low density lipoprotein receptor gene expression in T cells.
Non-sterol regulation of low density lipoprotein (LDL) receptor gene expression was examined in a mitogen-responsive human T cell line. Stimulation of the leukemic T cell line Jurkat with the phorbol ester phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore ionomycin rapidly and transiently increased LDL receptor mRNA levels. Inhibition of protein synthesis with cycloheximide (CHX) ...
متن کامل